D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T218S

Expression Data

Yield (mg/mL): 3.002

Kinetic Assay Data

**slope of T218S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.16E-01	2.10E-01	2.08E-01
B	25.00	1.18E-01	1.17E-01	1.17E-01
C	8.33	7.07E-02	6.95E-02	6.94E-02
D	2.78	3.64E-02	3.63E-02	3.52E-02
E	0.93	1.61E-02	1.57E-02	1.55E-02
F	0.31	5.65E-03	5.80E-03	5.85E-03
G	0.10	2.15E-03	2.35E-03	2.40E-03
H	0.03	9.50E-04	8.50E-04	8.50E-04

Yield (mg/mL): 3.002 Dilution Factor: 100×

Protein purified on 7/17/24 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-01 12:54:56.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.66E-01	1.65E-01	1.67E-01
B	25.00	1.25E-01	1.27E-01	1.21E-01
C	8.33	9.05E-02	9.22E-02	9.42E-02
D	2.78	5.56E-02	5.55E-02	5.69E-02
E	0.93	2.58E-02	2.57E-02	2.57E-02
F	0.31	1.00E-02	9.75E-03	9.70E-03
G	0.10	3.40E-03	3.35E-03	3.60E-03
H	0.03		1.40E-03	1.30E-03

Yield (mg/mL): 1.556 Dilution Factor: 100×

Protein purified on 7/17/24 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-01 12:57:30.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.