D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E388L

Expression Data

Yield (mg/mL): 2.188

Kinetic Assay Data

**slope of E388L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.86E-01	4.72E-01
B	25.00	4.04E-01	3.80E-01
C	8.33
D	2.78
E	0.93	4.21E-01	3.95E-01
F	0.31	1.66E-01	1.49E-01
G	0.10	5.08E-02
H	0.03	0	0

Yield (mg/mL): 2.188 Dilution Factor: 10×

Protein purified on 7/29/2024 and assayed on 7/30/2024.
Data uploaded by juanville and last updated on 2024-07-31 15:49:42.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.09E-01	2.27E-01	2.44E-01
B	25.00	1.44E-01	1.76E-01	1.80E-01
C	8.33	1.12E-01	1.19E-01	1.24E-01
D	2.78	7.95E-02		8.69E-02
E	0.93	7.92E-02	9.18E-02	8.18E-02
F	0.31	4.01E-02	3.00E-02	4.24E-02
G	0.10	2.23E-02	1.45E-02	2.50E-02
H	0.03	1.50E-04	1.50E-04

Yield (mg/mL): 2.682 Dilution Factor: 100×

Protein purified on 7/9/24 and assayed on 7/30/24.
Data uploaded by Azweiback and last updated on 2024-07-31 15:46:09.

Design Data

Rosetta Score for E388L: -0.37

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.