Raw Data for BglB Variant I238K

Expression Data

SDS-PAGE
 1.758

Kinetic Assay Data

slope of I238K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 11.726 9.3533 7.5554
B 25.00 7.3877 6.658 7.1971
C 8.33 4.9264 5.2152 4.906
D 2.78 2.3413 2.335 2.2794
E 0.93 1.10192 1.3455 1.3441
F 0.31 0.757 0.8054
G 0.10 0.2295 0.1941
H 0.03 0.0896 0.1108 0.1558
 1.758      10×

Protein purified on 4/16/21 and assayed on 4/16/21.
Data uploaded by hzhang79 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.139611765 0.139630294
B 25.00 0.128248971 0.119688676
C 8.33 0.086550882 0.086962794
D 2.78 0.039723529 0.043826029
E 0.93 0.022215 0.021438676
F 0.31 0.011845882 0.011395441
G 0.10 0.004566618 0.003670147
H 0.03 0.002452941 0.001793088
 0.83      100×

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of I238K A420 nm (min−1)
T (°C) 1 2
A 30.0 40.762 40.532
B 31.9 36.539 36.902
C 33.7 33.024 33.487
D 35.5 30.124 30.319
E 37.3 26.713 28.433
F 39.1 22.024 24.284
G 40.9 11.347 11.575
H 42.7 0.3281 0.403
I 44.5 0.0099 0.0165
J 46.3 0.0063 0.0091
K 48.1 0.0024 0.0084
L 50.0 0.0071 0.0053

Protein purified on 4/16/21 and assayed on 4/25/21.
Data uploaded by hzhang79 and last updated on 2022-07-28 15:35:07.


Reference Data for WT

slope of I238K A420 nm (min−1)
T (°C) 1 2
A 30.0 0.664885714
B 31.8 0.79277
C 33.6 0.77854
D 35.5 0.639442857
E 37.3 0.7241
F 39.1 0.67721
G 40.9 0.258779341
H 42.7 0.03094044
I 44.5 0.00119706
J 46.4 0.00043618
K 48.1 0.00025809
L 50.0 0.00022632

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-07-28 11:26:16.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.