D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N293V

Expression Data

Yield (mg/mL): 1.854

Kinetic Assay Data

**slope of N293V A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.09E-02	3.64E-02	5.52E-02
B	25.00		5.02E-02	4.33E-02
C	8.33	3.41E-02	2.81E-02	4.93E-02
D	2.78	2.18E-02		2.23E-02
E	0.93		1.13E-02	1.12E-02
F	0.31	6.00E-03	5.45E-03	5.50E-03
G	0.10	2.30E-03	3.10E-03	2.25E-03
H	0.03	3.00E-04	1.50E-04	2.50E-04

Yield (mg/mL): 1.854 Dilution Factor: 10×

Protein purified on 7/9/24 and assayed on 7/10/24.
Data uploaded by Azweiback and last updated on 2024-07-26 15:40:14.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.10E-01	1.95E-01	2.08E-01
B	25.00	1.93E-01	1.90E-01	1.83E-01
C	8.33	1.38E-01	1.49E-01	1.50E-01
D	2.78	9.79E-02	9.52E-02	9.61E-02
E	0.93	6.14E-02	5.14E-02	5.32E-02
F	0.31	2.23E-02	2.01E-02	2.81E-02
G	0.10	9.65E-03	7.65E-03	1.18E-02
H	0.03	3.00E-04	3.00E-04	3.00E-04

Yield (mg/mL): 2.682 Dilution Factor: 100×

Protein purified on 7/9/24 and assayed on 7/10/24.
Data uploaded by Azweiback and last updated on 2024-07-26 15:07:25.

Design Data

Rosetta Score for N293V: 7.749

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.