D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L171Q

Expression Data

Yield (mg/mL): 1.268

Kinetic Assay Data

**slope of L171Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.12E-01	2.12E-01	2.21E-01
B	25.00	1.70E-01	1.81E-01
C	8.33	1.39E-01	1.45E-01
D	2.78	9.34E-02	1.12E-01	9.24E-02
E	0.93	7.04E-02	6.37E-02	5.63E-02
F	0.31	3.69E-02	3.23E-02	3.18E-02
G	0.10	1.69E-02	1.51E-02	1.38E-02
H	0.03	3.00E-04		2.50E-04

Yield (mg/mL): 1.268 Dilution Factor: 100×

Protein purified on 7/9/24 and assayed on 7/10/24.
Data uploaded by Azweiback and last updated on 2024-07-29 13:47:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.10E-01	1.95E-01	2.08E-01
B	25.00	1.93E-01	1.90E-01	1.83E-01
C	8.33	1.38E-01	1.49E-01	1.50E-01
D	2.78	9.79E-02	9.52E-02	9.61E-02
E	0.93	6.14E-02	5.14E-02	5.32E-02
F	0.31	2.23E-02	2.01E-02	2.81E-02
G	0.10	9.65E-03	7.65E-03	1.18E-02
H	0.03	3.00E-04	3.00E-04	3.00E-04

Yield (mg/mL): 2.682 Dilution Factor: 100×

Protein purified on 7/9/24 and assayed on 7/10/24.
Data uploaded by Azweiback and last updated on 2024-07-26 15:07:25.

Design Data

Rosetta Score for L171Q: 3.94

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.