Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.22

Kinetic Assay Data

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.18E+02	2.33E+02	2.23E+02
	B	25.00	1.76E+02	1.76E+02	1.75E+02
	C	8.33	1.63E+02	1.54E+02	1.69E+02
	D	2.78	1.11E+02	1.26E+02	1.23E+02
	E	0.93	5.78E+01	6.83E+01	6.31E+01
	F	0.31	2.07E+01	2.48E+01	2.28E+01
	G	0.10	7.90E+00	9.30E+00	1.01E+01
	H	0.03	0		0

Yield (mg/mL): 3.22     Dilution Factor:  100×

Download these data

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by clmcintosh and last updated on 2024-07-25 20:01:22.

Thermostability Assay Data

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		T (°C)	1	2	3
	A	50.0		0	0
	B	48.3	0		0
	C	45.7	3.00E-01	3.00E-01
	D	42.4	3.26E+01	3.16E+01
	E	37.7	2.58E+02	2.41E+02	2.56E+02
	F	33.6	2.60E+02	2.62E+02	2.73E+02
	G	31.3	2.64E+02	2.74E+02	2.64E+02
	H	30.0	2.82E+02	2.79E+02	2.65E+02

Download these data

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by clmcintosh and last updated on 2024-07-25 20:06:58.

Citations

The unpublished data above were collected by students from South Mountain Community College. For inquiries, please contact Chelsea McIntosh: <chelsea.mcintosh@southmountaincc.edu>.