D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A80S

Yield (mg/mL): 3.19

**slope of A80S A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.60E+02	3.64E+02	3.71E+02
B	25.00	2.78E+02	2.74E+02	2.86E+02
C	8.33	2.50E+02	2.63E+02	2.20E+02
D	2.78	1.89E+02	1.91E+02	2.01E+02
E	0.93	1.13E+02	1.30E+02	1.59E+02
F	0.31	4.01E+01	4.77E+01	5.80E+01
G	0.10	1.45E+01	1.87E+01	2.36E+01
H	0.03		0	0

Yield (mg/mL): 3.19 Dilution Factor: 100×

Protein purified on 7/25/24 and assayed on 7/25/24.
Data uploaded by apak and last updated on 2024-07-25 19:48:10.

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.18E+02	2.33E+02	2.23E+02
B	25.00	1.76E+02	1.76E+02	1.75E+02
C	8.33	1.63E+02	1.54E+02	1.69E+02
D	2.78	1.11E+02	1.26E+02	1.23E+02
E	0.93	5.78E+01	6.83E+01	6.31E+01
F	0.31	2.07E+01	2.48E+01	2.28E+01
G	0.10	7.90E+00	9.30E+00	1.01E+01
H	0.03	0		0

Yield (mg/mL): 3.22 Dilution Factor: 100×

Protein purified on 7/25/24 and assayed on 7/25/24.
Data uploaded by apak and last updated on 2024-07-25 19:37:31.

**slope of A80S A_{420 nm} (10⁻³ min⁻¹)**
	T (°C)	1	2	3
A	50.0	5.00E-01	8.00E-01	6.00E-01
B	48.3	4.00E-01	5.00E-01
C	45.7	1.90E+00	1.80E+00	2.00E+00
D	42.4	1.32E+02	1.28E+02	1.27E+02
E	37.7	2.87E+02	3.16E+02	3.43E+02
F	33.6	3.71E+02	3.76E+02	3.69E+02
G	31.3	3.79E+02	3.63E+02	3.81E+02
H	30.0	3.89E+02	3.75E+02	3.78E+02

Protein purified on 7/25/24 and assayed on 7/25/24.
Data uploaded by apak and last updated on 2024-07-25 19:49:28.

**slope of A80S A_{420 nm} (10⁻³ min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	0		0
C	45.7	3.00E-01	3.00E-01
D	42.4	3.26E+01	3.16E+01
E	37.7	2.58E+02	2.41E+02	2.56E+02
F	33.6	2.60E+02	2.62E+02	2.73E+02
G	31.3	2.64E+02	2.74E+02	2.64E+02
H	30.0	2.82E+02	2.79E+02	2.65E+02

Protein purified on 7/25/24 and assayed on 7/25/24.
Data uploaded by apak and last updated on 2024-07-25 19:43:47.

Rosetta Score for A80S: 1.657

The unpublished data above were collected by students from Colorado School of Mines. For inquiries, please contact Alex Pak: <apak@mines.edu>.