Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.25

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.94E-01	1.79E-01	1.75E-01
	B	25.00	1.39E-01	1.48E-01	1.43E-01
	C	8.33	1.38E-01	1.21E-01	1.11E-01
	D	2.78	6.85E-02	7.25E-02	8.18E-02
	E	0.93	3.02E-02	3.41E-02	3.41E-02
	F	0.31	1.12E-02	1.27E-02	1.20E-02
	G	0.10	3.85E-03	3.95E-03	4.05E-03
	H	0.03	1.55E-03	1.60E-03	1.90E-03

Yield (mg/mL): 3.25     Dilution Factor:  100×

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Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ssilver and last updated on 2024-07-25 19:47:32.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		6.00E-04	6.00E-04
	B	48.3		8.00E-04	7.00E-04
	C	45.7	1.10E-03	7.00E-04	8.00E-04
	D	42.4	5.09E-02	4.30E-02	5.17E-02
	E	37.7	1.76E-01	1.63E-01	1.70E-01
	F	33.6	2.33E-01		2.27E-01
	G	31.3	2.25E-01	2.17E-01	2.08E-01
	H	30.0	2.18E-01	2.17E-01	2.00E-01

Download these data

Protein purified on 7/24/25 and assayed on 7/25/24.
Data uploaded by ssilver and last updated on 2024-07-25 19:50:41.

Citations

The unpublished data above were collected by students from Elmhurst University. For inquiries, please contact Sunshine Silver: <sunshine.silver@elmhurst.edu>.