Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.22

Kinetic Assay Data

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	218.2	233.2	222.6
	B	25.00	176.2	175.5	174.6
	C	8.33	162.7	154.2	168.7
	D	2.78	110.6	125.6	122.8
	E	0.93	57.8	68.3	63.1
	F	0.31	20.7	24.8	22.8
	G	0.10	7.9	9.3	10.1
	H	0.03	0	0.4	0.9

Yield (mg/mL): 3.22     Dilution Factor:  100×

Download these data

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by Jrichmond and last updated on 2024-07-25 19:48:22.

Thermostability Assay Data

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		T (°C)	1	2	3
	A	30.0	281.5	279.2	264.9
	B	31.3	263.9	273.5	264.1
	C	33.6	259.9	261.6	272.5
	D	37.7	258.4	240.8	255.9
	E	42.4	32.6	31.6
	F	45.7	0.3	0.3
	G	48.3	0		0
	H	50.0		0	0

Download these data

Protein purified on 7/24/2024 and assayed on 7/25/2025.
Data uploaded by Jrichmond and last updated on 2024-07-25 20:00:14.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Jennifer Richmond: <richmondj@yosemite.edu>.