Raw Data for BglB Variant W325K

Expression Data

SDS-PAGE
 1.539

Kinetic Assay Data

slope of W325K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00 4.63E-02 4.48E-02 4.51E-02
C 8.33 4.45E-02 4.23E-02 4.02E-02
D 2.78 2.27E-02 2.66E-02 2.55E-02
E 0.93 1.47E-02 1.37E-02 1.45E-02
F 0.31 5.35E-03 4.45E-03 4.75E-03
G 0.10 3.05E-03 2.95E-03 2.15E-03
H 0.03 9.00E-04 9.00E-04
 1.539      10×

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by montezano and last updated on 2024-07-25 19:41:13.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by montezano and last updated on 2024-07-25 19:56:47.


Thermostability Assay Data

slope of W325K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 6.00E-04
B 48.3 5.00E-04 6.00E-04
C 45.7 5.00E-04 4.00E-04
D 42.4 9.00E-04 9.00E-04 1.00E-03
E 37.7 2.20E-03 2.10E-03 1.80E-03
F 33.6 3.00E-03 2.70E-03 3.20E-03
G 31.3 3.00E-03 2.70E-03 3.00E-03
H 30.0 3.20E-03 3.10E-03 3.80E-03

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by montezano and last updated on 2024-07-25 19:48:58.


Reference Data for WT

slope of W325K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by montezano and last updated on 2024-07-25 20:00:11.


Citations

The unpublished data above were collected by students from . For inquiries, please contact Daniel Montezano: <montezano@ku.edu>.