D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F274N

Expression Data

Yield (mg/mL): 0.7

Kinetic Assay Data

**slope of F274N A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	34.7	35.8	31.4
B	25.00	29.7	29.8	28.7
C	8.33	23.9	22.8	23.1
D	2.78	20.7	18.7	20.8
E	0.93	12.3	11.7	9
F	0.31	5.6	4.9	4.3
G	0.10	1.9	1.9	2.3
H	0.03	0.5	0.2	0.8

Yield (mg/mL): 0.7 Dilution Factor: 100×

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by Jrichmond and last updated on 2024-07-25 19:40:12.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	218.2	233.2	222.6
B	25.00	176.2	175.5	174.6
C	8.33	162.7	154.2	168.7
D	2.78	110.6	125.6	122.8
E	0.93	57.8	68.3	63.1
F	0.31	20.7	24.8	22.8
G	0.10	7.9	9.3	10.1
H	0.03	0	0.4	0.9

Yield (mg/mL): 3.22 Dilution Factor: 100×

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by Jrichmond and last updated on 2024-07-25 19:48:22.

Thermostability Assay Data

**slope of F274N A_{420 nm} (10⁻³ min⁻¹)**
	T (°C)	1	2	3
A	30.0	27.9	29.9	29.7
B	31.3	24.9	25.6	27.1
C	33.6	18.8	19.8	20.1
D	37.7	4.7	5.1	5.2
E	42.4	0.3		0.3
F	45.7	0	0
G	48.3	0	0	0
H	50.0	0	0	0

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by Jrichmond and last updated on 2024-07-25 20:07:11.

Reference Data for WT

**slope of F274N A_{420 nm} (10⁻³ min⁻¹)**
	T (°C)	1	2	3
A	30.0	281.5	279.2	264.9
B	31.3	263.9	273.5	264.1
C	33.6	259.9	261.6	272.5
D	37.7	258.4	240.8	255.9
E	42.4	32.6	31.6
F	45.7	0.3	0.3
G	48.3	0		0
H	50.0		0	0

Protein purified on 7/24/2024 and assayed on 7/25/2025.
Data uploaded by Jrichmond and last updated on 2024-07-25 20:00:14.

Citations

The unpublished data above were collected by students from Modesto Junior College. For inquiries, please contact Jennifer Richmond: <richmondj@yosemite.edu>.