Raw Data for BglB Variant N220F

Expression Data

SDS-PAGE
 1.91

Kinetic Assay Data

slope of N220F A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 1.28E+02 1.30E+02 1.30E+02
B 25.00 1.16E+02 1.18E+02 1.22E+02
C 8.33 1.20E+02 1.23E+02 1.21E+02
D 2.78
E 0.93 1.21E+02 1.13E+02 1.24E+02
F 0.31 7.20E+01 7.46E+01 7.86E+01
G 0.10 3.54E+01 4.10E+01 4.70E+01
H 0.03 0
 1.91      100×

Protein purified on 7/24/24 and assayed on 7/24/24.
Data uploaded by clmcintosh and last updated on 2024-07-25 19:31:54.


Reference Data for WT

slope of WT A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 2.18E+02 2.33E+02 2.23E+02
B 25.00 1.76E+02 1.76E+02 1.75E+02
C 8.33 1.63E+02 1.54E+02 1.69E+02
D 2.78 1.11E+02 1.26E+02 1.23E+02
E 0.93 5.78E+01 6.83E+01 6.31E+01
F 0.31 2.07E+01 2.48E+01 2.28E+01
G 0.10 7.90E+00 9.30E+00 1.01E+01
H 0.03 0 0
 3.22      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by clmcintosh and last updated on 2024-07-25 20:01:22.


Thermostability Assay Data

slope of N220F A420 nm (10−3 min−1)
T (°C) 1 2 3
A 50.0 0
B 48.3 2.00E-01 2.00E-01
C 45.7 7.00E-01 6.00E-01 6.00E-01
D 42.4 7.10E+00 8.50E+00 8.50E+00
E 37.7 1.35E+02 1.31E+02 1.26E+02
F 33.6 1.99E+02 1.66E+02 2.21E+02
G 31.3 2.13E+02 2.13E+02 2.10E+02
H 30.0 2.28E+02 2.21E+02 2.22E+02

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by clmcintosh and last updated on 2024-07-25 20:10:54.


Reference Data for WT

slope of N220F A420 nm (10−3 min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0 0
C 45.7 3.00E-01 3.00E-01
D 42.4 3.26E+01 3.16E+01
E 37.7 2.58E+02 2.41E+02 2.56E+02
F 33.6 2.60E+02 2.62E+02 2.73E+02
G 31.3 2.64E+02 2.74E+02 2.64E+02
H 30.0 2.82E+02 2.79E+02 2.65E+02

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by clmcintosh and last updated on 2024-07-25 20:06:58.


Citations

The unpublished data above were collected by students from South Mountain Community College. For inquiries, please contact Chelsea McIntosh: <chelsea.mcintosh@southmountaincc.edu>.