D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T133Y

Expression Data

Yield (mg/mL): 3.224

Kinetic Assay Data

**slope of T133Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.46E-01	2.57E-01
B	25.00	2.22E-01	1.99E-01	2.11E-01
C	8.33
D	2.78	1.65E-01	1.52E-01	1.31E-01
E	0.93	9.43E-02	8.30E-02	8.97E-02
F	0.31	3.85E-02	4.45E-02	4.29E-02
G	0.10	1.98E-02	1.83E-02	1.87E-02
H	0.03	0	0

Yield (mg/mL): 3.224 Dilution Factor: 100×

Protein purified on 7/10/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:20:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.76E-01	1.69E-01
B	25.00		1.33E-01	1.39E-01
C	8.33
D	2.78	1.03E-01	1.10E-01	1.13E-01
E	0.93		6.52E-02	6.21E-02
F	0.31	4.24E-02	3.34E-02	2.75E-02
G	0.10	1.80E-02	1.39E-02	1.02E-02
H	0.03	2.50E-04	2.50E-04

Yield (mg/mL): 2.14 Dilution Factor: 100×

Protein purified on 7/10/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:25:41.

Design Data

Rosetta Score for T133Y: 6.801

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.