D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120E

Expression Data

Yield (mg/mL): 0.932

Kinetic Assay Data

**slope of W120E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.57E-02	1.30E-02	1.38E-02
B	25.00	5.25E-03	4.55E-03
C	8.33
D	2.78	1.90E-03	2.00E-03	2.15E-03
E	0.93	1.60E-03	1.55E-03	1.40E-03
F	0.31	8.00E-04	7.00E-04	6.00E-04
G	0.10	6.50E-04	5.50E-04	4.00E-04
H	0.03	0		0

Yield (mg/mL): 0.932 Dilution Factor: 10×

Protein purified on 7/10/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:22:25.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.76E-01	1.69E-01
B	25.00		1.33E-01	1.39E-01
C	8.33
D	2.78	1.03E-01	1.10E-01	1.13E-01
E	0.93		6.52E-02	6.21E-02
F	0.31	4.24E-02	3.34E-02	2.75E-02
G	0.10	1.80E-02	1.39E-02	1.02E-02
H	0.03	2.50E-04	2.50E-04

Yield (mg/mL): 2.14 Dilution Factor: 100×

Protein purified on 7/10/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:25:41.

Design Data

Rosetta Score for W120E: 4.684

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.