Raw Data for BglB Variant E377Q

Expression Data

SDS-PAGE
 0.306

Kinetic Assay Data

slope of E377Q A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.132 0.1392 0.1458
B 25.00 0.1058 0.1111 0.1165
C 8.33 0.072 0.0791 0.0833
D 2.78 0.0486 0.0587 0.0651
E 0.93 0.0238 0.0241 0.0246
F 0.31 0.0213 0.0203 0.0223
G 0.10 0.0102 0.0088 0.0091
H 0.03 0.0029 0.0028 0.0028
 0.306      100×

Protein purified on 4/16/2021 and assayed on 4/24/2021.
Data uploaded by magnle01 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.114 0.120
B 25.00 0.0790 0.0784
C 8.33 0.0304 0.0836
D 2.78 0.0467 0.0489
E 0.93 0.0407 0.0330
F 0.31 0.0141 0.0139
G 0.10 0.00520 0.00360
H 0.03 0.00460 0.00330
 0.834      100×

Protein purified on 4/14/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of E377Q A420 nm (min−1)
T (°C) 1 2
A 30.0 0.3965 0.4516
B 31.8 0.5808 0.5762
C 33.6 0.5825 0.5741
D 35.5 0.5245 0.4733
E 37.3 0.5396 0.4793
F 39.1 0.3316 0.3954
G 40.9 0.129 0.1231
H 42.7 0.0065 0.0075
I 44.5 0.0014 0.0015
J 46.4 0.0006 0.0007
K 48.1 0.0005 0.0005
L 50.0 0.0004 0.0005

Protein purified on 4/16/2021 and assayed on 4/24/2021.
Data uploaded by magnle01 and last updated on 2022-07-28 15:05:15.


Reference Data for WT

slope of E377Q A420 nm (min−1)
T (°C) 1 2
A 30.0 6.65E-01
B 31.8 7.93E-01
C 33.6 7.79E-01
D 35.5 0.639
E 37.3 0.724
F 39.1 0.677
G 40.9 0.259
H 42.7 0.0309
I 44.5 0.0012
J 46.4 0.0004
K 48.1 0.0003
L 50.0 0.0002

Protein purified on 4/14/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-07-28 13:47:07.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.