Raw Data for BglB Variant I91K

Expression Data

SDS-PAGE
 0.306

Kinetic Assay Data

slope of I91K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.356432727 0.323954545 0.357064242
B 25.00 0.273186813 0.263989231
C 8.33 0.35995697 0.305954895 0.253534286
D 2.78 0.207626029 0.170718676 0.120033676
E 0.93 0.015884118 0.020593529 0.013393529
F 0.31 0.005854706 0.007257059 0.006121765
G 0.10 0.0017625 0.002604853 0.003127941
H 0.03 0.001160147 0.001213088
 0.306      100×

Protein purified on 4/16/21 and assayed on 2/24/21.
Data uploaded by Gyasu and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.139611765 0.139630294
B 25.00 0.128248971 0.119688676
C 8.33 0.086550882 0.086962794
D 2.78 0.039723529 0.043826029
E 0.93 0.022215 0.021438676
F 0.31 0.011845882 0.011395441
G 0.10 0.004566618 0.003670147
H 0.03 0.002452941 0.001793088
 0.83      100×

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of I91K A420 nm (min−1)
T (°C) 1 2
A 30.0 0.425411667
B 31.8 0.354853636 0.383527273
C 33.6 0.350483636 0.36924
D 35.5 0.336222727 0.345265455
E 37.3 0.347087273 0.300571978
F 39.1 0.328671818 0.329381818
G 40.9 0.120644265 0.1107225
H 42.7 0.002159265 0.002224118
I 44.5 0.000218676 0.000143382
J 46.4 0.000100441 5.88235E-07
K 48.1 0.000217206 3.98529E-05
L 50.0 0.0001275 0

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-07-28 11:26:11.


Reference Data for WT

slope of I91K A420 nm (min−1)
T (°C) 1 2
A 30.0 0.664885714
B 31.8 0.79277
C 33.6 0.77854
D 35.5 0.639442857
E 37.3 0.7241
F 39.1 0.67721
G 40.9 0.258779341
H 42.7 0.03094044
I 44.5 0.00119706
J 46.4 0.00043618
K 48.1 0.00025809
L 50.0 0.00022632

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-07-28 11:26:16.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.