D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A236S

Expression Data

Yield (mg/mL): 0.13

Kinetic Assay Data

**slope of A236S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.40E-04	1.80E-04
B	25.00		6.00E-05	6.00E-05
C	8.33	5.00E-05		6.00E-05
D	2.78	7.00E-05	3.00E-05
E	0.93	3.00E-05
F	0.31
G	0.10
H	0.03

Yield (mg/mL): 0.13 Dilution Factor: 10×

Protein purified on 4/22/24 and assayed on 4/24/24.
Data uploaded by mvlouie and last updated on 2024-07-08 14:47:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.54E-01	3.66E-01	3.67E-01
B	25.00	2.76E-01	2.61E-01	2.80E-01
C	8.33	1.95E-01	1.99E-01	2.04E-01
D	2.78	1.11E-01	1.17E-01	1.17E-01
E	0.93	5.07E-02	5.31E-02	5.28E-02
F	0.31	1.93E-02	1.99E-02	2.13E-02
G	0.10	7.07E-03	7.73E-03	7.67E-03
H	0.03	2.93E-03	3.44E-03	3.85E-03

Yield (mg/mL): 3.35 Dilution Factor: 100×

Protein purified on 4/22/24 and assayed on 5/14/24.
Data uploaded by mvlouie and last updated on 2024-07-03 13:00:13.

Design Data

Rosetta Score for A236S: 6.653

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.