Raw Data for BglB Variant S298R

Expression Data

SDS-PAGE
 0.761

Kinetic Assay Data

slope of S298R A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.026319 0.136218 0.0844
B 25.00 0.132583 0.080899 0.103113
C 8.33 0.089157 0.05684 0.072137
D 2.78 0.061753 0.043011 0.04956
E 0.93 0.02326 0.017639 0.021118
F 0.31 0.016917 0.01206 0.016315
G 0.10 0.004245 0.003556
H 0.03 0.002071 0.000902 0.001604
 0.761      100×

Protein purified on 4/16/2021 and assayed on 4/24/2021.
Data uploaded by strobjt and last updated on 2023-01-11 13:22:41.


Thermostability Assay Data

slope of S298R A420 nm (min−1)
T (°C) 1 2
A 30.0 2.90E-01 2.64E-01
B 31.8 3.74E-01 3.33E-01
C 33.6 4.54E-01 3.70E-01
D 35.5 0.409135 0.350168182
E 37.3 0.380670909 0.381095152
F 39.1 0.262775357 0.239228676
G 40.9 0.020935882 0.018941765
H 42.7 0.002451176 0.002520441
I 44.5 0.001052794 0.000729559
J 46.4 0.000245441 0.000283382
K 48.1 0.000476176 0.000270588
L 50.0 7.77941E-05 0.000194706

Protein purified on 4/16/2021 and assayed on 4/24/2021.
Data uploaded by strobjt and last updated on 2022-07-28 15:04:58.


Reference Data for WT

slope of S298R A420 nm (min−1)
T (°C) 1 2
A 30.0 6.65E-01
B 31.8 7.40E-01
C 33.6 7.16E-01
D 35.5 0.639442857
E 37.3 0.660682857
F 39.1 0.636757143
G 40.9 0.253220882
H 42.7 0.030940441
I 44.5 0.001197059
J 46.4 0.000436176
K 48.1 0.000258088
L 50.0 0.000226324

Protein purified on 4/14/2021 and assayed on 4/24/2021.
Data uploaded by strobjt and last updated on 2022-07-28 15:05:02.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.