Raw Data for BglB Variant N220Q

Expression Data

Kinetic Assay Data

slope of N220Q A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	7.83E-02	7.91E-02	7.95E-02
	B	25.00	5.70E-02	5.69E-02	6.24E-02
	C	8.33	3.80E-02	5.13E-02	5.12E-02
	D	2.78	2.84E-02	3.03E-02	2.70E-02
	E	0.93	9.75E-03	1.01E-02	9.90E-03
	F	0.31	3.75E-03	4.05E-03	3.75E-03
	G	0.10	1.80E-03	1.95E-03	1.80E-03
	H	0.03	7.50E-04		7.50E-04

Yield (mg/mL): 6.385     Dilution Factor:  100×

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Protein purified on 3/27/2023 and assayed on 3/27/2023.
Data uploaded by mlope618 and last updated on 2024-06-26 15:29:21.

Thermostability Assay Data

slope of N220Q A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.50E-04	6.00E-04
	B	48.3
	C	45.7	9.00E-04	7.50E-04	7.50E-04
	D	42.4	6.00E-04	9.00E-04	7.50E-04
	E	37.7	1.58E-02	1.58E-02	1.41E-02
	F	33.6	3.33E-02	3.27E-02	3.21E-02
	G	31.3	3.89E-02	3.84E-02	3.32E-02
	H	30.0	4.11E-02	4.37E-02	3.60E-02

Download these data

Protein purified on 3/27/2023 and assayed on 3/27/2023.
Data uploaded by mlope618 and last updated on 2024-08-08 14:49:57.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.