D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L219Q

Expression Data

Yield (mg/mL): 0.5

Kinetic Assay Data

**slope of L219Q A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	35.9300	25.2980	14.0750
B	25.00	37.1170	21.9980	5.2550
C	8.33	10.4150	11.9190	9.7790
D	2.78	14.7310	6.8350	10.1850
E	0.93	6.77100	9.39300	7.20000
F	0.31
G	0.10	1.087000	1.402000	1.228000
H	0.03	0.866000	0.855000	0.684000

Yield (mg/mL): 0.5 Dilution Factor: 100×

Protein purified on 5/13/2023 and assayed on 5/15/2024.
Data uploaded by amaduros and last updated on 2024-06-27 10:56:00.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	556.6180	437.9750
B	25.00	409.9050	324.9950	515.4950
C	8.33		293.7130	315.1380
D	2.78		305.2660	273.1950
E	0.93	215.71300	163.40200	174.95200
F	0.31	151.88100	79.35900	102.00000
G	0.10	61.068000	25.816000	38.924000
H	0.03	36.447000	12.921000	20.144000

Yield (mg/mL): 2.226 Dilution Factor: 100×

Protein purified on 5/13/2023 and assayed on 5/15/2023.
Data uploaded by amaduros and last updated on 2024-12-19 18:47:48.

Design Data

Rosetta Score for L219Q: 6.613

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.