D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D288E

Expression Data

Yield (mg/mL): 2.416

Kinetic Assay Data

**slope of D288E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.83E-01	1.83E-01	1.84E-01
B	25.00	1.59E-01	1.66E-01	1.58E-01
C	8.33	1.69E-01	1.62E-01	1.41E-01
D	2.78	7.80E-02	9.66E-02	8.08E-02
E	0.93	3.40E-02		3.30E-02
F	0.31	1.32E-02	1.56E-02	1.37E-02
G	0.10	5.45E-03	5.60E-03	5.45E-03
H	0.03		2.50E-04	2.50E-04

Yield (mg/mL): 2.416 Dilution Factor: 100×

Protein purified on 6/17/2024 and assayed on 6/18/2024.
Data uploaded by HollyTruong and last updated on 2024-06-24 14:24:19.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.39E-01	1.45E-01	1.43E-01
B	25.00	1.37E-01	1.38E-01	1.32E-01
C	8.33	1.38E-01	1.46E-01	1.24E-01
D	2.78	8.32E-02	7.12E-02	9.26E-02
E	0.93	3.45E-02	3.23E-02	3.42E-02
F	0.31	1.48E-02	1.21E-02	1.25E-02
G	0.10	5.90E-03	4.45E-03	3.95E-03
H	0.03	1.50E-04	0	2.00E-04

Yield (mg/mL): 2.268 Dilution Factor: 100×

Protein purified on 6/17/2024 and assayed on 7/18/2024.
Data uploaded by HollyTruong and last updated on 2024-06-24 12:48:40.

Design Data

Rosetta Score for D288E: 3.567

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.