Raw Data for BglB Variant E388Y

Expression Data

SDS-PAGE
 4.217

Kinetic Assay Data

slope of E388Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78
E 0.93 2.09E-01 2.32E-01
F 0.31 1.69E-01 1.38E-01 1.39E-01
G 0.10 7.30E-02 6.30E-02 7.20E-02
H 0.03 3.75E-02 3.65E-02 3.70E-02
 4.217      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by tcris and last updated on 2024-06-13 19:19:36.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 2.08E-01
E 0.93 1.44E-01 1.25E-01 1.55E-01
F 0.31 7.50E-02 8.25E-02 1.00E-01
G 0.10 3.85E-02 4.80E-02 4.85E-02
H 0.03 2.10E-02 1.65E-02 2.65E-02
 5.636      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 15:39:04.


Thermostability Assay Data

slope of E388Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.00E-01 3.00E-01
B 48.3 3.00E-01 3.00E-01
C 45.7 1.00E+00 8.00E-01
D 42.4 7.00E+00 7.00E+00 7.90E+00
E 37.7 2.72E+02 2.23E+02 2.35E+02
F 33.6 5.15E+02 6.35E+02 4.44E+02
G 31.3 6.16E+02 5.56E+02 3.89E+02
H 30.0 7.13E+02 5.24E+02

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by tcris and last updated on 2024-06-13 19:27:10.


Reference Data for WT

slope of E388Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.30
C 45.7 0.80 0.80 0.90
D 42.4 75.90 79.40
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 17:24:00.


Design Data

35.697

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.