Raw Data for BglB Variant E388Y

Expression Data

SDS-PAGE
 3.988

Kinetic Assay Data

slope of E388Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 1.71E-01 1.48E-01 1.63E-01
E 0.93 6.70E-02 6.10E-02 7.10E-02
F 0.31 2.50E-02 2.30E-02 2.80E-02
G 0.10 7.50E-03 6.50E-03 9.00E-03
H 0.03 3.50E-03 3.50E-03
 3.988      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by tcris and last updated on 2024-06-13 17:54:27.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78
E 0.93 1.77E-01 1.69E-01 1.45E-01
F 0.31 6.05E-02 5.95E-02 5.45E-02
G 0.10 2.25E-02 2.15E-02 1.75E-02
H 0.03 7.00E-03 6.50E-03 7.00E-03
 6.66      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 15:20:29.


Thermostability Assay Data

slope of E388Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.40 0.40
B 48.3 0.30 0.40
C 45.7 0.40 0.30 0.20
D 42.4 5.70 6.50 6.80
E 37.7 241.60 297.20 267.60
F 33.6 460.50 433.40
G 31.3 487.60 462.20 447.60
H 30.0 492.60 366.70 548.20

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by tcris and last updated on 2024-06-13 18:06:39.


Reference Data for WT

slope of E388Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.40
C 45.7 1.30 1.20 0.90
D 42.4 59.90 73.50 70.60
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:21:19.


Design Data

35.697

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.