Raw Data for BglB Variant Q139E

Expression Data

SDS-PAGE
 5.54

Kinetic Assay Data

slope of Q139E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78
E 0.93 2.23E-01
F 0.31 1.51E-01 1.41E-01
G 0.10 1.24E-01 9.50E-02
H 0.03 4.05E-02 3.70E-02
 5.54      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by Ben and last updated on 2024-06-13 15:53:24.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 2.08E-01
E 0.93 1.44E-01 1.25E-01 1.55E-01
F 0.31 7.50E-02 8.25E-02 1.00E-01
G 0.10 3.85E-02 4.80E-02 4.85E-02
H 0.03 2.10E-02 1.65E-02 2.65E-02
 5.636      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 15:39:04.


Thermostability Assay Data

slope of Q139E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.40 0.50 0.40
B 48.3 0.50 0.50
C 45.7 1.70 1.50
D 42.4 125.80 122.20
E 37.7 704.30
F 33.6 678.20 710.40
G 31.3 781.60
H 30.0 631.60

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by Ben and last updated on 2024-06-13 17:31:44.


Reference Data for WT

slope of Q139E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.30
C 45.7 0.80 0.80 0.90
D 42.4 75.90 79.40
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 17:24:00.


Design Data

-0.563

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.