Raw Data for BglB Variant N293V

Expression Data

SDS-PAGE
 5.294

Kinetic Assay Data

slope of N293V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.50E-02 1.10E-02 1.25E-02
B 25.00 1.05E-02 1.20E-02 1.10E-02
C 8.33 1.00E-02 9.50E-03 1.10E-02
D 2.78 7.00E-03 7.00E-03 6.50E-03
E 0.93 4.00E-03 4.00E-03 4.00E-03
F 0.31 2.50E-03 2.00E-03 2.00E-03
G 0.10 1.00E-03 1.00E-03
H 0.03 5.00E-04 5.00E-04
 5.294      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by epei679 and last updated on 2024-06-13 15:43:40.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 2.08E-01
E 0.93 1.44E-01 1.25E-01 1.55E-01
F 0.31 7.50E-02 8.25E-02 1.00E-01
G 0.10 3.85E-02 4.80E-02 4.85E-02
H 0.03 2.10E-02 1.65E-02 2.65E-02
 5.636      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 15:39:04.


Thermostability Assay Data

slope of N293V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.30 0.20
B 48.3 0 0.40 0.90
C 45.7 0.40 0 0.70
D 42.4 0.50 0.40 0.40
E 37.7 9.20 8.90
F 33.6 8.50 12.00 11.00
G 31.3 13.30 13.60 10.70
H 30.0 13.00 11.20 11.40

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by epei679 and last updated on 2024-06-13 19:10:48.


Reference Data for WT

slope of N293V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.30
C 45.7 0.80 0.80 0.90
D 42.4 75.90 79.40
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 17:24:00.


Design Data

1.015

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.