Raw Data for BglB Variant Q139E

Expression Data

SDS-PAGE
 5.137

Kinetic Assay Data

slope of Q139E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78
E 0.93 1.06E-01 1.08E-01
F 0.31 3.80E-02 3.90E-02
G 0.10 1.30E-02 1.35E-02
H 0.03 5.00E-03 5.00E-03
 5.137      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by Ben and last updated on 2024-06-13 15:44:26.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 1.63E-01 2.10E-01
E 0.93 1.34E-01 8.85E-02
F 0.31 4.25E-02 2.90E-02
G 0.10 1.70E-02 1.10E-02
H 0.03 6.50E-03 4.00E-03
 5.558      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 14:25:41.


Thermostability Assay Data

slope of Q139E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.70 0.50
C 45.7 1.70 3.20
D 42.4 139.70 114.60 77.30
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by Ben and last updated on 2024-06-13 17:27:29.


Reference Data for WT

slope of Q139E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.40
C 45.7 0.90 0.80 0.80
D 42.4 53.70 56.00
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:17:54.


Design Data

-0.563

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.