Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.636

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00
	B	25.00
	C	8.33
	D	2.78	2.08E-01
	E	0.93	1.44E-01	1.25E-01	1.55E-01
	F	0.31	7.50E-02	8.25E-02	1.00E-01
	G	0.10	3.85E-02	4.80E-02	4.85E-02
	H	0.03	2.10E-02	1.65E-02	2.65E-02

Yield (mg/mL): 5.636     Dilution Factor:  100×

Download these data

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 15:39:04.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.30	0.30
	B	48.3		0.30	0.30
	C	45.7	0.80	0.80	0.90
	D	42.4	75.90		79.40
	E	37.7
	F	33.6
	G	31.3
	H	30.0

Download these data

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by jerengregory and last updated on 2024-06-13 17:24:00.

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.