D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325M

Expression Data

Yield (mg/mL): 1.472

Kinetic Assay Data

**slope of W325M A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	19.2000	5.7000	15.8000
B	25.00	1.3000	8.5000	15.4000
C	8.33	10.3000		11.6000
D	2.78	0.7000	2.8000	5.5000
E	0.93	1.00000	1.20000
F	0.31	2.00000	0.80000	1.50000
G	0.10	0.400000	0.500000
H	0.03	0.500000		0.500000

Yield (mg/mL): 1.472 Dilution Factor: 100×

Protein purified on 5/13/2024 and assayed on 5/15/2024.
Data uploaded by lfarinsky and last updated on 2024-06-27 10:34:13.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	220.9000	259.9000	254.0000
B	25.00	202.7000	207.8000	200.4000
C	8.33	145.6000	137.3000	124.9000
D	2.78	34.8000	34.1000	39.2000
E	0.93	16.40000	8.80000	14.10000
F	0.31	5.00000	4.00000	5.40000
G	0.10	2.100000	2.400000	3.000000
H	0.03	0.900000	0.800000	0.900000

Yield (mg/mL): 2.016 Dilution Factor: 100×

Protein purified on 5/13/2024 and assayed on 5/15/2024.
Data uploaded by lfarinsky and last updated on 2024-06-27 10:41:06.

Design Data

Rosetta Score for W325M: 3.455

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.