Raw Data for BglB Variant N220T

Expression Data

Kinetic Assay Data

slope of N220T A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.19E-02	1.19E-02	1.20E-02
	B	25.00	8.85E-03	8.70E-03	8.10E-03
	C	8.33	5.85E-03	5.40E-03	5.10E-03
	D	2.78	2.70E-03	2.85E-03	3.15E-03
	E	0.93	1.35E-03	1.50E-03	1.20E-03
	F	0.31	1.05E-03	1.05E-03	9.00E-04
	G	0.10		7.50E-04	9.00E-04
	H	0.03	0		0

Yield (mg/mL): 4.29     Dilution Factor:  4×

Download these data

Protein purified on 5/24/2024 and assayed on 5/24/2024.
Data uploaded by mlope618 and last updated on 2024-05-24 15:56:06.

Thermostability Assay Data

slope of N220T A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	55.0	0.0006000	0.0006000	0.0006000
	B	53.3	0.0007500		0.0006000
	C	50.7	0	0
	D	47.3	0		0
	E	42.7	0.001500	0.001500	0.001350
	F	38.7	0.006900	0.006900	0.007050
	G	36.3	0.009600	0.008850	0.008400
	H	35.0	0.009750	0.009600	0.01005

Download these data

Protein purified on 5/31/2024 and assayed on 5/21/2024.
Data uploaded by mlope618 and last updated on 2024-05-31 14:19:42.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.