Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.128	0.137
	B	25.00	0.130	0.129
	C	8.33	0.110	0.100
	D	2.78	0.080	0.086
	E	0.93	0.065	0.047
	F	0.31	0.040	0.031
	G	0.10	0.025	0.016
	H	0.03		0

Yield (mg/mL): 1.38     Dilution Factor:  100×

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Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	0.38
	B	31.8	0.4374
	C	33.6	0.3474
	D	35.5	0.3655
	E	37.3	0.3835
	F	39.1	0.3743
	G	40.9	0.22
	H	42.7	0.0204
	I	44.5	0.0008
	J	46.4	0.0003
	K	48.1	0
	L	50.0	0.0003

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Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-07-28 11:05:05.

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.