Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.89

Kinetic Assay Data

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.76E+02	2.68E+02	2.95E+02
	B	25.00	2.44E+02	2.43E+02	2.46E+02
	C	8.33	2.36E+02	2.62E+02	2.54E+02
	D	2.78	1.80E+02	1.60E+02	1.79E+02
	E	0.93	6.16E+01	6.37E+01	5.80E+01
	F	0.31	4.75E-01	2.83E+01	3.83E+01
	G	0.10	1.25E+01	1.39E+01	1.20E+01
	H	0.03	5.36E+00	5.34E+00	5.51E+00

Yield (mg/mL): 0.89     Dilution Factor:  100×

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Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by dlivira and last updated on 2024-05-22 14:34:47.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		3.96E-01	3.48E-01
	B	48.3		3.72E-01	7.52E-01
	C	45.7	1.93E+00	1.73E+00	2.46E+00
	D	42.4	1.10E+02	1.09E+02	1.36E+02
	E	37.7	3.65E+02	3.37E+02	3.56E+02
	F	33.6	4.10E+02	3.62E+02	2.54E+02
	G	31.3	3.63E+02	3.55E+02	3.55E+02
	H	30.0	3.53E+02	3.64E+02	3.66E+02

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Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by dlivira and last updated on 2024-05-22 14:42:57.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.