Raw Data for BglB Variant W409S

Expression Data

SDS-PAGE
 0.723

Kinetic Assay Data

slope of W409S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00 8.54E-02 7.87E-02 8.42E-02
C 8.33
D 2.78 8.27E-02 6.46E-02 8.63E-02
E 0.93 4.49E-02 5.71E-02 4.80E-02
F 0.31 2.66E-02 3.47E-02 3.31E-02
G 0.10 1.28E-02 1.61E-02 1.59E-02
H 0.03 4.51E-03 5.71E-03 6.86E-03
 0.723      10×

Protein purified on 4/15/21 and assayed on 4/24/21.
Data uploaded by winkju01 and last updated on 2023-01-11 13:11:29.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.102 0.092
B 25.00 0.0822 0.0765
C 8.33 0.0718 0.0765
D 2.78 0.0406 0.0412
E 0.93 0.0163 0.0197
F 0.31 0.0086 0.0076
G 0.10 0.0028 0.0034
H 0.03 0.001 0.0009
 0.83      100×

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-07-28 16:16:53.


Thermostability Assay Data

slope of W409S A420 nm (min−1)
T (°C) 1 2
A 30.0 4.85E-01 6.23E-01
B 31.8 8.13E-01 5.47E-01
C 33.6 5.30E-01 5.68E-01
D 35.5 0.431432143 0.5248
E 37.3 0.4596 0.50537
F 39.1 0.314116364 0.410260714
G 40.9 0.078949706 0.094365441
H 42.7 0.001324412 0.001973529
I 44.5 0.000496912 0.000481176
J 46.4 0.000186765 0.000235882
K 48.1 0 5.92647E-05
L 50.0 0 5.33824E-05

Protein purified on 4/15/21 and assayed on 4/24/21.
Data uploaded by winkju01 and last updated on 2022-07-28 15:04:47.


Reference Data for WT

slope of W409S A420 nm (min−1)
T (°C) 1 2
A 30.0 0.38
B 31.8 0.4374
C 33.6 0.3474
D 35.5 0.3655
E 37.3 0.3835
F 39.1 0.3743
G 40.9 0.22
H 42.7 0.0204
I 44.5 0.0008
J 46.4 0.0003
K 48.1 0
L 50.0 0.0003

Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-07-28 11:05:05.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.