Raw Data for BglB Variant D150V

Expression Data

SDS-PAGE
 0.38

Kinetic Assay Data

slope of D150V A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 0.09735 0.08850 0.1101
B 25.00 0.09130 0.1024 0.09460
C 8.33 0.1292 0.07655
D 2.78 0.04520 0.04810 0.04350
E 0.93 0.02015 0.02080 0.02135
F 0.31 0.008100 0.008050 0.008150
G 0.10 0.003700 0.003350 0.003750
H 0.03 0.002200 0.002950 0.001750
 0.38      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by tyousefi and last updated on 2024-06-05 17:36:35.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.4104 0.3731 0.4015
B 25.00 0.2936 0.3667 0.3656
C 8.33 0.3006 0.2905 0.2660
D 2.78 0.1869 0.1915 0.1908
E 0.93 0.08815 0.09025 0.08730
F 0.31 0.03495 0.03495 0.03200
G 0.10 0.013100 0.014900 0.013900
H 0.03 0.006750 0.006300 0.008400
 1.37      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:56:57.


Thermostability Assay Data

slope of D150V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.003000 0.004300 0.002500
B 48.3 0.001000 0.001000 0.0008000
C 45.7 0.002300 0.001500 0.0008000
D 42.4 0.005800 0.006000 0.005199
E 37.7 0.08660 0.09740 0.09210
F 33.6 0.09310 0.1022 0.09910
G 31.3 0.09990 0.09530 0.09010
H 30.0 0.1085 0.1020 0.1089

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by tyousefi and last updated on 2024-05-22 14:43:24.


Reference Data for WT

slope of D150V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00150 0 0.000500
B 48.3 0.0025000 0.001800 0.0020000
C 45.7 0.010800 0.010200
D 42.4 0.12360 0.11550
E 37.7 0.4620 0.4222 0.4359
F 33.6 0.5037 0.4569 0.4778
G 31.3 0.4306 0.4816 0.4612
H 30.0 0.4487 0.4498 0.4508

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:58:20.


Design Data

19.017

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.