D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H328P

Expression Data

Yield (mg/mL): 0.36

Kinetic Assay Data

**slope of H328P A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.0130	4.6150	3.3860
B	25.00	4.3370	4.8750
C	8.33	4.1900	3.6230
D	2.78	2.1430	2.0620
E	0.93	0.93200	0.93900
F	0.31	0.38600		0.50000
G	0.10	0		0
H	0.03		0.774000	0

Yield (mg/mL): 0.36 Dilution Factor: 10×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by amandalastimado and last updated on 2024-05-22 11:28:35.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	422.86	461.595	435.472
B	25.00	372.049	374.071	345.585
C	8.33	344.404	334.833	346.619
D	2.78	222.817	196.032	261.951
E	0.93		159.434	156.977
F	0.31	54.931	74.107	102.037
G	0.10	23.077	26.324
H	0.03	9.445	10.588

Yield (mg/mL): 1.21 Dilution Factor: 100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by lolareimbold and last updated on 2024-05-22 11:17:06.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of H328P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.001200	0.001600	0.001000
B	48.3	0.002100	0.001600	0.002000
C	45.7	0.003100	0.003800	0.005200
D	42.4	0.1133	0.09210	0.1034
E	37.7	0.2491	0.2723
F	33.6	0.3178	0.3007	0.2445
G	31.3	0.2765	0.2451	0.1971
H	30.0	0.2345	0.2210	0.2038

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by lolareimbold and last updated on 2024-05-22 11:26:09.

Design Data

Rosetta Score for H328P: 50.989

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.