Raw Data for BglB Variant Q124R

Expression Data

SDS-PAGE
 0.35

Kinetic Assay Data

slope of Q124R A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.54E-02 5.23E-02 4.94E-02
B 25.00 3.60E-02 4.93E-02 4.40E-02
C 8.33 3.38E-02 2.73E-02 3.23E-02
D 2.78 2.54E-02 2.49E-02 2.27E-02
E 0.93 9.30E-03 9.50E-03
F 0.31
G 0.10 2.35E-03 2.45E-03
H 0.03 1.60E-03 1.05E-03 1.20E-03
 0.35      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by JaydenCosta24 and last updated on 2024-05-22 11:44:29.


Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.



Thermostability Assay Data

slope of Q124R A420 nm (min−1)
T (°C) 1 2 3
A 50.0 2.10E-03 1.70E-03 1.10E-03
B 48.3 8.00E-04 8.00E-04
C 45.7 1.10E-03 9.00E-04
D 42.4 3.10E-03 3.30E-03 3.50E-03
E 37.7 1.76E-02 1.89E-02 2.05E-02
F 33.6 2.50E-02 2.86E-02
G 31.3 3.67E-02 2.74E-02 3.27E-02
H 30.0 4.29E-02 3.31E-02 3.40E-02

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by JaydenCosta24 and last updated on 2024-05-22 11:39:27.



Design Data

5.407


Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.