D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D241R

Expression Data

Yield (mg/mL): 0.1349

Kinetic Assay Data

**slope of D241R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.90E-03		2.05E-03
B	25.00	1.90E-03	1.95E-03	1.70E-03
C	8.33	1.45E-03	1.90E-03	1.50E-03
D	2.78	1.05E-03	1.00E-03	8.50E-04
E	0.93	5.50E-04	7.00E-04
F	0.31	3.50E-04	4.00E-04
G	0.10			4.00E-04
H	0.03

Yield (mg/mL): 0.1349 Dilution Factor: 10×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by mrflynn and last updated on 2024-05-22 11:14:57.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.05E-01	3.13E-01	3.09E-01
B	25.00	3.05E-01	2.78E-01	2.81E-01
C	8.33		1.45E-01	1.38E-01
D	2.78		7.85E-02	7.37E-02
E	0.93		2.20E-02	1.55E-02
F	0.31		2.49E-02	2.12E-02
G	0.10		4.90E-03	4.00E-03
H	0.03		9.50E-04	8.00E-04

Yield (mg/mL): 1.04 Dilution Factor: 100×

Protein purified on 4/24/24 and assayed on 5/1/24.
Data uploaded by nschen and last updated on 2024-05-22 11:34:00.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of D241R A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.0044	0	1.50E-03
B	48.3	2.20E-03	1.20E-03	3.00E-03
C	45.7	1.10E-03	1.80E-03	1.60E-03
D	42.4	7.14E-02	8.61E-02	8.66E-02
E	37.7	2.46E-01	2.46E-01	2.50E-01
F	33.6	2.58E-01	2.47E-01	2.43E-01
G	31.3	2.83E-01	2.92E-01	2.52E-01
H	30.0	2.69E-01	2.82E-01	2.66E-01

Protein purified on 4/24/24 and assayed on 5/8/24.
Data uploaded by nschen and last updated on 2024-05-22 11:37:14.

Design Data

Rosetta Score for D241R: 1.255

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.