D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R372H

Yield (mg/mL): 3.568

**slope of R372H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.53E-01	6.74E-01	6.82E-01
B	25.00	5.41E-01	5.31E-01	5.46E-01
C	8.33	4.43E-01	4.37E-01	4.29E-01
D	2.78	2.89E-01	2.66E-01	2.94E-01
E	0.93	1.69E-01	1.83E-01	1.66E-01
F	0.31	5.52E-02	5.08E-02	4.71E-02
G	0.10	2.05E-02	2.03E-02	1.94E-02
H	0.03	7.65E-03	7.95E-03	6.80E-03

Yield (mg/mL): 3.568 Dilution Factor: 100×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-16 17:12:12.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.96E-01	2.85E-01	2.72E-01
B	25.00	2.40E-01	2.47E-01	2.28E-01
C	8.33	1.86E-01	1.74E-01	1.81E-01
D	2.78	1.37E-01	1.42E-01	1.58E-01
E	0.93	7.40E-02	8.14E-02	7.14E-02
F	0.31	2.50E-02	2.51E-02	2.69E-02
G	0.10	9.60E-03	1.05E-02	8.85E-03
H	0.03	3.75E-03	3.55E-03	3.25E-03

Yield (mg/mL): 1.626 Dilution Factor: 100×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-16 17:07:29.

Rosetta Score for R372H: 2.863

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.