Raw Data for BglB Variant N220D

Expression Data

 1.164

Kinetic Assay Data

slope of N220D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.036 0.029
B 25.00 0.035 0.035
C 8.33 0.032 0.029
D 2.78 0.019 0.019
E 0.93 0.014 0.012
F 0.31 0.008 0.009
G 0.10 0.007 0.007
H 0.03 0 0 0
 1.164      100×

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.128 0.137
B 25.00 0.130 0.129
C 8.33 0.110 0.100
D 2.78 0.080 0.086
E 0.93 0.065 0.047
F 0.31 0.040 0.031
G 0.10 0.025 0.016
H 0.03 0
 1.38      100×

Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of N220D A420 nm (min−1)
T (°C) 1 2
A 30.0 0.170 0.1397
B 31.8 1.47E-01 0.1261
C 33.6 1.26E-01 0.097
D 35.5 0.0923 0.0985
E 37.3 0.0217 0.0247
F 39.1 0.0007 0.0005
G 40.9 0.00009 0.000008
H 42.7 0.0001 0.0001
I 44.5 0 0
J 46.4 0 0
K 48.1 0 0
L 50.0 0 0

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-07-28 11:04:59.


Reference Data for WT

slope of N220D A420 nm (min−1)
T (°C) 1 2
A 30.0 0.38
B 31.8 0.4374
C 33.6 0.3474
D 35.5 0.3655
E 37.3 0.3835
F 39.1 0.3743
G 40.9 0.22
H 42.7 0.0204
I 44.5 0.0008
J 46.4 0.0003
K 48.1 0
L 50.0 0.0003

Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-07-28 11:05:05.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.