Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.68E-01	1.79E-01	1.78E-01
	B	25.00	1.40E-01	1.56E-01	1.45E-01
	C	8.33	1.12E-01	1.13E-01	1.11E-01
	D	2.78	7.05E-02	6.77E-02	6.34E-02
	E	0.93	3.37E-02	2.96E-02	2.94E-02
	F	0.31	1.14E-02	1.16E-02	1.24E-02
	G	0.10	4.05E-03		4.35E-03
	H	0.03	2.50E-04	2.00E-04	1.50E-04

Yield (mg/mL): 0.901     Dilution Factor:  100×

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Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by mheisler and last updated on 2024-05-16 11:34:38.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.00E+00	0.00E+00
	B	48.3	0.00E+00	4.00E-04
	C	45.7		5.00E-04	6.00E-04
	D	42.7	2.54E-02	2.52E-02	3.18E-02
	E	37.7	1.61E-01	1.63E-01	1.57E-01
	F	33.6	1.91E-01	1.75E-01	1.74E-01
	G	31.3	1.74E-01	1.78E-01	1.79E-01
	H	30.0	1.84E-01	1.79E-01	1.81E-01

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Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by SamN and last updated on 2024-05-16 11:40:46.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.