Raw Data for BglB Variant W409T
Expression Data
0.118
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 3.25E-03 | 3.10E-03 | 2.65E-03 | ||
| B | 25.00 | 2.65E-03 | 2.35E-03 | 1.85E-03 | ||
| C | 8.33 | 1.60E-03 | 1.45E-03 | 1.70E-03 | ||
| D | 2.78 | 1.40E-03 | 1.25E-03 | 9.50E-04 | ||
| E | 0.93 | 6.00E-04 | 6.00E-04 | 5.50E-04 | ||
| F | 0.31 | 4.00E-04 | 4.00E-04 | 3.00E-04 | ||
| G | 0.10 | 2.50E-04 | 2.50E-04 | |||
| H | 0.03 | 2.50E-04 | 2.00E-04 | 1.00E-04 |
Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by christineduffy24 and last updated on 2024-05-16 11:23:16.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.68E-01 | 1.79E-01 | 1.78E-01 | ||
| B | 25.00 | 1.40E-01 | 1.56E-01 | 1.45E-01 | ||
| C | 8.33 | 1.12E-01 | 1.13E-01 | 1.11E-01 | ||
| D | 2.78 | 7.05E-02 | 6.77E-02 | 6.34E-02 | ||
| E | 0.93 | 3.37E-02 | 2.96E-02 | 2.94E-02 | ||
| F | 0.31 | 1.14E-02 | 1.16E-02 | 1.24E-02 | ||
| G | 0.10 | 4.05E-03 | 4.35E-03 | |||
| H | 0.03 | 2.50E-04 | 2.00E-04 | 1.50E-04 |
Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by mheisler and last updated on 2024-05-16 11:34:38.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 6.00E-04 | 5.00E-04 | |||
| B | 48.3 | 0.00E+00 | 0.00E+00 | 0.00E+00 | ||
| C | 45.7 | 0.00E+00 | 0.00E+00 | |||
| D | 42.4 | 4.00E-04 | 4.00E-04 | |||
| E | 37.7 | 7.00E-04 | 7.00E-04 | |||
| F | 33.6 | 9.00E-04 | 1.10E-03 | 9.00E-04 | ||
| G | 31.3 | 1.10E-03 | 1.30E-03 | |||
| H | 30.0 | 2.10E-03 | 0 | 1.50E-03 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 0.00E+00 | 0.00E+00 | |||
| B | 48.3 | 0.00E+00 | 4.00E-04 | |||
| C | 45.7 | 5.00E-04 | 6.00E-04 | |||
| D | 42.7 | 2.54E-02 | 2.52E-02 | 3.18E-02 | ||
| E | 37.7 | 1.61E-01 | 1.63E-01 | 1.57E-01 | ||
| F | 33.6 | 1.91E-01 | 1.75E-01 | 1.74E-01 | ||
| G | 31.3 | 1.74E-01 | 1.78E-01 | 1.79E-01 | ||
| H | 30.0 | 1.84E-01 | 1.79E-01 | 1.81E-01 |
Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by SamN and last updated on 2024-05-16 11:40:46.
Design Data
Citations
The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.