Raw Data for BglB Variant W409I

Expression Data

SDS-PAGE
 1.123

Kinetic Assay Data

slope of W409I A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.54E-01 2.42E-01 2.25E-01
B 25.00 1.26E-01 1.37E-01
C 8.33 1.32E-01 1.28E-01 1.24E-01
D 2.78 6.94E-02 6.98E-02 6.48E-02
E 0.93 3.05E-02 2.90E-02 2.58E-02
F 0.31 1.03E-02 1.04E-02 9.50E-03
G 0.10 3.80E-03 3.80E-03
H 0.03 5.00E-04 6.00E-04 7.00E-04
 1.123      100×

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by Gonzalezsa and last updated on 2024-05-16 14:11:48.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.18E-01 1.17E-01 1.10E-01
B 25.00 1.01E-01 8.86E-02 8.72E-02
C 8.33 6.06E-02 5.76E-02 4.79E-02
D 2.78 2.94E-02 2.96E-02 2.81E-02
E 0.93 1.13E-02 1.09E-02 1.09E-02
F 0.31 4.00E-03 3.90E-03 3.60E-03
G 0.10 1.50E-03 1.60E-03 1.50E-03
H 0.03 0.00E+00 5.00E-04 8.00E-04
 0.752      100×

Protein purified on 4/11/2024 and assayed on 4/20/2024.
Data uploaded by KeairaC and last updated on 2024-05-16 11:30:43.


Thermostability Assay Data

slope of W409I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 6.00E-04 4.00E-04
B 48.3 4.00E-04 0.00E+00 6.00E-04
C 45.7 9.00E-04 8.00E-04 6.00E-04
D 42.4 3.80E-02 3.67E-02 3.18E-02
E 37.7 2.26E-01 2.30E-01 2.38E-01
F 33.6 2.23E-01 2.21E-01 2.28E-01
G 31.3 2.43E-01 2.46E-01 2.52E-01
H 30.0 2.52E-01 2.54E-01 2.35E-01

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by AvaRNewton and last updated on 2024-05-16 11:26:46.


Reference Data for WT

slope of W409I A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 6.00E-04
B 48.3 3.00E-04 0.00E+00 4.00E-04
C 45.7 5.00E-04 6.00E-04 7.00E-04
D 42.4 2.66E-02 2.65E-02 3.02E-02
E 37.7 1.36E-01 1.31E-01 1.31E-01
F 33.6 1.40E-01 1.36E-01
G 31.3 1.38E-01 1.35E-01 1.34E-01
H 30.0 1.44E-01 1.44E-01

Protein purified on 4/11/2024 and assayed on 4/20/2024.
Data uploaded by KeairaC and last updated on 2024-05-16 11:40:39.


Design Data

5.715

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.