D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S331T

Expression Data

Yield (mg/mL): 0.638

Kinetic Assay Data

**slope of S331T A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.15E+02	1.08E+02	1.18E+02
B	25.00	1.17E+02	1.22E+02	1.44E+02
C	8.33	9.97E+01	1.01E+02	8.33E+01
D	2.78	5.50E+01	8.17E+01	3.65E+01
E	0.93	1.82E+01		1.60E+01
F	0.31	9.07E+00		1.02E+01
G	0.10	4.64E+01	7.73E+00
H	0.03	1.68E+00		1.20E+00

Yield (mg/mL): 0.638 Dilution Factor: 10×

Protein purified on 2024/5/6 and assayed on 2024/5/7.
Data uploaded by YuanAngela666 and last updated on 2024-05-15 16:01:18.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.12E+03	1.03E+03	9.20E+02
B	25.00	9.08E+02	8.17E+02	7.83E+02
C	8.33	7.50E+02	6.18E+02	5.73E+02
D	2.78	4.17E+02	3.56E+02	3.82E+02
E	0.93	2.21E+02	1.88E+02	1.72E+02
F	0.31	8.94E+01	6.70E+01	6.51E+01
G	0.10	3.61E+01	2.22E+01	2.76E+01
H	0.03	1.04E+01	6.16E+00	8.31E+00

Yield (mg/mL): 4.168 Dilution Factor: 100×

Protein purified on 2024/5/6 and assayed on 2024/5/7.
Data uploaded by YuanAngela666 and last updated on 2024-05-14 15:54:07.

Design Data

Rosetta Score for S331T: 45.028

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.