D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P429A

Expression Data

Yield (mg/mL): 2.398

Kinetic Assay Data

**slope of P429A A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.90E+02	5.37E+02	7.04E+02
B	25.00		5.35E+02	6.16E+02
C	8.33		3.69E+02	3.49E+02
D	2.78	2.50E+02	2.24E+02	1.76E+02
E	0.93		7.26E+01	7.89E+01
F	0.31	7.69E+01	3.74E+01	6.63E+01
G	0.10		1.44E+01	1.35E+01
H	0.03		4.41E+00	5.17E+00

Yield (mg/mL): 2.398 Dilution Factor: 100×

Protein purified on 2024/5/6 and assayed on 2024/5/8.
Data uploaded by YuanAngela666 and last updated on 2024-05-15 15:57:05.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.12E+03	1.03E+03	9.20E+02
B	25.00	9.08E+02	8.17E+02	7.83E+02
C	8.33	7.50E+02	6.18E+02	5.73E+02
D	2.78	4.17E+02	3.56E+02	3.82E+02
E	0.93	2.21E+02	1.88E+02	1.72E+02
F	0.31	8.94E+01	6.70E+01	6.51E+01
G	0.10	3.61E+01	2.22E+01	2.76E+01
H	0.03	1.04E+01	6.16E+00	8.31E+00

Yield (mg/mL): 4.168 Dilution Factor: 100×

Protein purified on 2024/5/6 and assayed on 2024/5/7.
Data uploaded by YuanAngela666 and last updated on 2024-05-14 15:54:07.

Design Data

Rosetta Score for P429A: 3.851

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.