Raw Data for BglB Variant Q19A

Expression Data

SDS-PAGE
 1.248

Kinetic Assay Data

slope of Q19A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0006500 0.0007500
B 25.00 0.0004500 0.0003500 0.0004000
C 8.33 0.0003500 0.0002500 0.0002000
D 2.78 0.0002500 0.0002000
E 0.93 0.0002500 0.0002500 0.0002000
F 0.31 0.0002000 0.0002000
G 0.10 0.0002000 0 0.0001500
H 0.03 0.0002000 0.0002500 0.0002000
 1.248      10×

Protein purified on 4/10/2024 and assayed on 4/17/2024.
Data uploaded by PeterLochow and last updated on 2024-05-15 11:28:49.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.33E-01 2.16E-01 2.26E-01
B 25.00 1.74E-01 1.77E-01 1.82E-01
C 8.33 1.36E-01 1.29E-01 1.28E-01
D 2.78 8.24E-02 7.99E-02 7.69E-02
E 0.93 3.63E-02 3.55E-02 3.60E-02
F 0.31 1.43E-02 1.52E-02 1.34E-02
G 0.10 5.00E-03 5.15E-03 5.00E-03
H 0.03 0 0
 0.862      100×

Protein purified on 4/10/2024 and assayed on 4/17/2024.
Data uploaded by YvonneP and last updated on 2024-05-15 11:33:33.


Thermostability Assay Data

slope of Q19A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.00E-04 3.00E-04
B 48.3 3.50E-04 3.50E-04
C 45.7 6.00E-04 6.50E-04 6.00E-04
D 42.4 2.69E-02 2.23E-02
E 37.7 3.43E-01 3.77E-01
F 33.6 1.93E-01 4.89E-01 8.18E-01
G 31.3 8.75E-01 8.68E-01
H 30.0 9.11E-01 1.01E+00 9.48E-01

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by ejaneckova and last updated on 2024-05-16 17:44:23.


Reference Data for WT

slope of Q19A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.50E-04 2.50E-04 2.00E-04
B 48.3 2.50E-04 2.50E-04
C 45.7 5.50E-04 3.50E-04 5.00E-04
D 42.4 1.91E-02 2.00E-02 2.31E-02
E 37.7 1.96E-01 1.62E-01 9.21E-02
F 33.6 1.70E-01 1.67E-01
G 31.3 1.75E-01 1.81E-01 2.10E-01
H 30.0 1.96E-01 1.84E-01 1.81E-01

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by ejaneckova and last updated on 2024-05-16 17:47:18.


Design Data

-1.28

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Eva Janeckova: <ejaneckova@miracosta.edu>.