D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220M

Expression Data

Yield (mg/mL): 0.533

Kinetic Assay Data

**slope of N220M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.88E-02	5.98E-02	5.50E-02
B	25.00	5.01E-02	5.50E-02	5.53E-02
C	8.33	4.93E-02	3.97E-02	4.65E-02
D	2.78	3.29E-02	3.59E-02	3.71E-02
E	0.93	1.94E-02	2.03E-02	2.11E-02
F	0.31	8.95E-03	9.70E-03	1.00E-02
G	0.10	4.00E-03	3.85E-03	3.75E-03
H	0.03	0	1.50E-04	2.00E-04

Yield (mg/mL): 0.533 Dilution Factor: 100×

Protein purified on 4/10/2024 and assayed on 4/17/2024.
Data uploaded by YvonneP and last updated on 2024-05-15 11:32:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.33E-01	2.16E-01	2.26E-01
B	25.00	1.74E-01	1.77E-01	1.82E-01
C	8.33	1.36E-01	1.29E-01	1.28E-01
D	2.78	8.24E-02	7.99E-02	7.69E-02
E	0.93	3.63E-02	3.55E-02	3.60E-02
F	0.31	1.43E-02	1.52E-02	1.34E-02
G	0.10	5.00E-03	5.15E-03	5.00E-03
H	0.03		0	0

Yield (mg/mL): 0.862 Dilution Factor: 100×

Protein purified on 4/10/2024 and assayed on 4/17/2024.
Data uploaded by YvonneP and last updated on 2024-05-15 11:33:33.

Thermostability Assay Data

**slope of N220M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	3.00E-04	7.50E-04	5.00E-04
B	48.3	6.00E-04	0	2.00E-04
C	45.7	7.00E-04		7.50E-04
D	42.4	2.70E-03	2.90E-03
E	37.7	1.75E-02	2.39E-02	1.45E-02
F	33.6	6.19E-02	4.86E-02	4.41E-02
G	31.3	5.66E-02	6.81E-02	8.04E-02
H	30.0	6.18E-02	6.49E-02	7.80E-02

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by ejaneckova and last updated on 2024-05-16 17:57:25.

Reference Data for WT

**slope of N220M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	3.50E-04	2.50E-04	2.00E-04
B	48.3	2.50E-04		2.50E-04
C	45.7	5.50E-04	3.50E-04	5.00E-04
D	42.4	1.91E-02	2.00E-02	2.31E-02
E	37.7	1.96E-01	1.62E-01	9.21E-02
F	33.6		1.70E-01	1.67E-01
G	31.3	1.75E-01	1.81E-01	2.10E-01
H	30.0	1.96E-01	1.84E-01	1.81E-01

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by ejaneckova and last updated on 2024-05-16 17:47:18.

Design Data

Rosetta Score for N220M: 1.037

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Eva Janeckova: <ejaneckova@miracosta.edu>.