D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E406Q

Expression Data

Yield (mg/mL): 0.905

Kinetic Assay Data

**slope of E406Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.55E-03	3.40E-03	3.50E-03
B	25.00	1.60E-03	1.70E-03	1.65E-03
C	8.33	8.50E-04	8.00E-04	6.50E-04
D	2.78	4.00E-04	3.50E-04	3.50E-04
E	0.93	3.00E-04	2.50E-04	3.00E-04
F	0.31	1.50E-04	2.00E-04	2.00E-04
G	0.10	0.00E+00		0.00E+00
H	0.03	0.00E+00		0.00E+00

Yield (mg/mL): 0.905 Dilution Factor: 10×

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by ShawnBio and last updated on 2024-05-15 11:22:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.14E-01	2.16E-01	2.08E-01
B	25.00	1.86E-01	1.77E-01	1.76E-01
C	8.33	1.30E-01	1.31E-01	1.30E-01
D	2.78	7.21E-02	7.65E-02	7.64E-02
E	0.93	3.14E-02	3.34E-02	3.42E-02
F	0.31	1.30E-02	1.36E-02	1.56E-02
G	0.10	5.85E-03	6.00E-03	6.05E-03
H	0.03	2.50E-04	2.00E-04

Yield (mg/mL): 0.905 Dilution Factor: 10×

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by ShawnBio and last updated on 2024-05-15 11:37:23.

Design Data

Rosetta Score for E406Q: 4.305

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Eva Janeckova: <ejaneckova@miracosta.edu>.