D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q139E

Expression Data

Yield (mg/mL): 4.168

Kinetic Assay Data

**slope of Q139E A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.08E+02	8.58E+02	8.60E+02
B	25.00	8.35E+02	6.71E+02	6.30E+02
C	8.33	4.74E+02	5.80E+02	5.82E+02
D	2.78	2.09E+02	2.04E+02
E	0.93	1.15E+02	1.90E+02	2.60E+02
F	0.31	4.75E+01	5.69E+01
G	0.10		3.14E+01	7.95E+01
H	0.03	5.65E+00	7.18E+00	8.87E+00

Yield (mg/mL): 4.168 Dilution Factor: 100×

Protein purified on 2024/5/6 and assayed on 2024/5/7.
Data uploaded by YuanAngela666 and last updated on 2024-05-14 15:39:19.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.12E+03	1.03E+03	9.20E+02
B	25.00	9.08E+02	8.17E+02	7.83E+02
C	8.33	7.50E+02	6.18E+02	5.73E+02
D	2.78	4.17E+02	3.56E+02	3.82E+02
E	0.93	2.21E+02	1.88E+02	1.72E+02
F	0.31	8.94E+01	6.70E+01	6.51E+01
G	0.10	3.61E+01	2.22E+01	2.76E+01
H	0.03	1.04E+01	6.16E+00	8.31E+00

Yield (mg/mL): 4.168 Dilution Factor: 100×

Protein purified on 2024/5/6 and assayed on 2024/5/7.
Data uploaded by YuanAngela666 and last updated on 2024-05-14 15:54:07.

Design Data

Rosetta Score for Q139E: -0.555

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.