Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00		9.03E-03	9.25E-03
	B	25.00		7.02E-03	6.47E-03
	C	8.33		3.98E-03	4.23E-03
	D	2.78		2.79E-03	2.26E-03
	E	0.93		1.35E-03	1.04E-03
	F	0.31		1.95E-04	3.67E-04
	G	0.10	1.06E-03	7.58E-04	2.07E-05
	H	0.03	4.13E-04	1.51E-04	0

Yield (mg/mL): 0.504     Dilution Factor:  100×

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Protein purified on 4/9/2024 and assayed on 4/11/2024.
Data uploaded by jacksonjones and last updated on 2024-04-22 22:02:32.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	1.32E-02	1.16E-02	1.25E-02
	B	31.3	1.20E-02	1.27E-02	1.03E-02
	C	33.6	1.16E-02	1.22E-02	9.44E-03
	D	37.7	6.30E-03	5.29E-03	6.89E-03
	E	42.4		6.20E-03	6.62E-03
	F	45.7	3.62E-04	5.14E-05	2.25E-04
	G	48.3	1.38E-04	9.85E-05	1.28E-04
	H	50.0	2.34E-04	1.66E-04	4.26E-05

Download these data

Protein purified on 4/9/2024 and assayed on 4/11/2024.
Data uploaded by jacksonjones and last updated on 2024-04-22 21:31:52.

Citations

The unpublished data above were collected by students from University of Alabama, Huntsville. For inquiries, please contact Sharifa: <str0012@uah.edu>.