Raw Data for BglB Variant H379Y

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of H379Y A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.71E-03	5.49E-03	8.99E-03
	B	48.7	5.83E-03		6.22E-03
	C	46.2	3.40E-03	6.12E-03	4.84E-03
	D	42.3	5.77E-03		6.34E-03
	E	37.7		1.49E-02	1.48E-02
	F	33.9	1.29E-02	1.73E-02	1.48E-02
	G	31.4	1.48E-02		1.36E-02
	H	30.0	1.19E-02	1.03E-02	1.21E-02

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by vwjohnson and last updated on 2024-04-18 12:07:11.

Reference Data for WT

slope of H379Y A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	7.89E-03	8.36E-03
	B	48.7	4.70E-03	6.05E-03	6.95E-03
	C	46.2	3.75E-03		4.34E-03
	D	42.3	3.32E-02	4.03E-02	3.34E-02
	E	37.7	1.13E-01	1.35E-01	1.36E-01
	F	33.9	1.15E-01	1.27E-01	1.31E-01
	G	31.4	1.22E-01	1.34E-01	1.27E-01
	H	30.0	1.03E-01	1.25E-01	1.29E-01

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by slneilson and last updated on 2024-04-11 12:35:48.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.