D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H182T

Expression Data

Yield (mg/mL): 4.016

Kinetic Assay Data

**slope of H182T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33
D	2.78	9.84E-01	9.66E-01	9.61E-01
E	0.93	4.63E-01	4.64E-01	4.77E-01
F	0.31	1.55E-01	1.56E-01	1.50E-01
G	0.10	5.70E-02	5.25E-02	4.80E-02
H	0.03		5.00E-04	5.00E-04

Yield (mg/mL): 4.016 Dilution Factor: 10×

Protein purified on 3/28/24 and assayed on 4/11/24.
Data uploaded by TreySci and last updated on 2024-04-18 11:50:45.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		6.46E-01	7.20E-01
B	25.00	5.61E-01	5.75E-01	5.38E-01
C	8.33	4.44E-01	4.10E-01	3.84E-01
D	2.78	2.10E-01	2.44E-01	2.27E-01
E	0.93	9.55E-02	9.20E-02	9.90E-02
F	0.31	3.00E-02	3.50E-02	3.50E-02
G	0.10	1.00E-02	1.20E-02	1.25E-02
H	0.03	1.48E-16	1.48E-16	1.48E-16

Yield (mg/mL): 5.018 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by NoahSmithUNF2024 and last updated on 2024-04-18 11:21:02.

Design Data

Rosetta Score for H182T: 8.116

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.